TABLE 3.
Results of multiplex PCR test on groups of E. coli representing different phenotypes
| Group | Phenotypic characterization | Serogroup | Host species | Genotypic characterizationa | PCR results (no. in agreement/no. tested) |
|---|---|---|---|---|---|
| a | ETEC | O101, O9 | Calf and pig | K99, F41, STa | 9/9 |
| O8 | Calf | K99, STa | 2/2 | ||
| O9 | Pig | F41, STa | 1/1 | ||
| O9, O20, O141, O148, O149, O78 | Pig and human | STa | 8/8 | ||
| b | EPEC | O55, O111, O119, O125, O126, O127, O128, O142 | Human | Intimin | 16/16 |
| c | STEC | O157:H7, O157 NM, O15, O17 | Calf and human | Intimin, Stx1, Stx2 | 19/19 |
| O5, O84, O103, O133 | Calf | Intimin, Stx1 | 10/10 | ||
| d | Various | O101 | Calf | F41 | 1/1 |
| O9 | Calf | F41, K99 | 1/1 | ||
| O133, OX3, O22 | Calf | Stx1, Stx2 | 3/3 | ||
| O98 | Pig | Stx1 | 1/1 | ||
| O5, O8, O171 | Calf | Stx2 | 3/3 | ||
| O139 | Pig | Stx2e | 1/1 | ||
| O8, O138 | Pig | Stx2e, STa | 3/3 | ||
| O119 | Calf and pig | Stx2, STa | 1/1 | ||
| e | Nonpathogens | Calf and pig | No genes of interest | 11/11 |
a
Genes encoding the indicated virulence factors were determined previously by DNA colony blot hybridization.