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. Author manuscript; available in PMC: 2024 Aug 9.
Published in final edited form as: Cell Host Microbe. 2023 Jul 28;31(8):1371–1385.e6. doi: 10.1016/j.chom.2023.07.003

Figure 1. Aer01 associates with host intestinal mucus and aggregates in response to its mucolytic activity.

Figure 1.

(A-C) Confocal microscopy of fixed 5 dpf larval zebrafish intestine indicating Aer01 (anti-dTom, magenta) and mucus (WGA-488, green) distribution (a) throughout the entire larval zebrafish gut, (b) the fore/mid-gut, and (c) distal mid-gut region. See also Figure S1.

(D) Experimental design of culture-based aggregation assay and calculation of aggregation index.

(E) Top: representative images of mucin-and GlcNAc-mediated Aer01 aggregation after 6 hr exposure. Bottom: mean aggregation index of Aer01 exposed to PGM or its monomeric glycan components (N=3).

(F) Aer01 hydrolysis of 4-Nitrophenyl (pNP) substrates. (N=5). The mean values are indicated in all figures.