Table 1.

Key enzymes suggested for efficient degradation of O-acetyl-galactoglucomannan

Enzyme class (EC number)	CAZyme family	Substrate Specificity	References
AcME (EC 3.1.1.72)	CE2, 16	Active on 3-O-, 4-O- and 6-O-acetylations on hetero-mannans	(Bååth et al. 2018)
	CE17	Active on 2-O-acetylations, including double substituted oligomers	(Michalak et al. 2020)
Aga (EC 3.2.1.22)	GH27	Active on both short MOS and mannans substituted with D-galactose residues	(Malgas et al. 2015b; Coconi Linares et al. 2020)
	GH4, 36	Active on short MOS substituted with D-galactose residues	(Malgas et al. 2015b; Coconi Linares et al. 2020)
BGL (EC 3.2.1.21)	GH1, 3	Active in terminal, non-reducing D-glucosyl residues derived from glucomannan	(Bai et al. 2021)
CBM	CBM1	Affinity towards cellulose	(von Freiesleben et al. 2016; Freiesleben et al. 2018; Uechi et al. 2020)
	CBM35	Affinity towards mannans	(von Freiesleben et al. 2018)
EXP	-	Disruptor of cellulose-hemicellulose association	(Zhu et al. 2022)
LPMO (EC 1.14.99.54/56)	AA9, AA10	Disruption of GM-celluose complexes and oxidative cleavage of carbohydrates	(Sun et al. 2023)
MBH (EC 3.2.1.100)	GH26	Non-reducing end specific exo-mannanase removes successive mannobiose residues from mannan	(Cartmell et al. 2008; Reddy et al. 2016)
Mnd (EC 3.2.1.25)	GH1, 2, 164	Active on terminal, nonreducing D-mannose residues in short MOS (higher specificity with decreasing DP)	(Hsu and Arioka 2020; Armstrong and Davies 2020; Couturier et al. 2022)
	GH5	Active on terminal, non-reducing D-mannose residues in long MOS (higher specificity with increasing DP)	(Malgas et al. 2022)
MAN (EC 3.2.1.78)	GH5	Active on glucomannan and insoluble mannan	(Tailford et al. 2009)
	GH26	Active in GalM and soluble mannan	(Tailford et al. 2009)
	GH45	Active in GM and cellulose	(Kirsch et al. 2012; Busch et al. 2019)
	GH113, 134	Active on linear mannan	(You et al. 2018a, b)
Swol	-	Disruptor of cellulose-hemicellulose association	(Herburger et al. 2020; Gu et al. 2021; Zhu et al. 2022)