Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 9;80(10):288. doi: 10.1007/s00018-023-04928-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — The TGFβ1 pathway is overactivated in MMSCs isolated from rats neonatally exposed to EDC. Real-time PCR analysis of mRNAs, protein levels and representative gels of A LTBP1, B THBS1, and C TGFβ1 (arrows indicate band for: Pro-TGFβ1 at 44 kDa, Dimer of mature TGFβ1 at 25 kDa, and Monomer of mature TGFβ1 at 12.5 kDa) in VEH- and EDC-MMSCs isolated from 5-month-old rats. D TGFβ1 levels in culture supernatants collected from VEH- and EDC-MMSCs cultures. Immunofluorescence images of E TGFβ1 and F TGFβ RI in VEH- and EDC-MMSCs. Scale bar 20 µm. The white arrows indicate cells in mitotic stages expressing TGFβ1 or TGFβ RI. F mRNAs levels of Smad2 and Smad3 in VEH- and EDC-MMSCs isolated from 5-month-old rats. G Representative gel of p-Smad2 and Smad2 in VEH- and EDC-MMSCs. mRNA data were normalized by the amount of 18S and protein levels by the amount of β-actin. Data are shown as mean ± S.E.M. from triplicate data. ns not significant. *p < 0.05, ***p < 0.001, ****p < 0.0001, Student’s t test. EDC endocrine-disrupting chemical, MMCSs myometrial stem cells, THBS1 thrombospondin 1, LTBP1 latent TGFβ binding protein 1, TGFβ1 transforming growth factor beta 1, TGFβ RI transforming growth factor beta receptor 1, UtSM human uterine smooth muscle cell line. TGFβ1 treatment: 10 ng/ml for 1 h (positive control)