Table 3.
MBC values for each agent on each bacterial strain
| 2.3 Agar disc diffusion (inhibition zone, MV ± SD, mm) | 2.4 Broth dilution (μL of agent, 70 mmol/L, that eradicated bacteria after 5/10 min) | 2.5 Broth microdilution on agar plates (MBC, mmol/L) | 2.6 Efficacy of active chlorine ** | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAR | PER | NOG | OCL | AAP | DAK | H2O2 | CHX | CAR | PER | DAK | CHX | H2O2 | CAR | PER | DAK | CHX | H2O2 | CAR 5 min |
CAR 10 min |
PER 5 min |
PER 10min |
|
| Lactobacillus paracasei CCUG 32212 | 7.0 ± 0.7 | 6.7 ± 0.2 | 6.5 ± 0.0 | 9.3 ± 0.9 | 6.0 ± 0.0 | 10.3 ± 0.3 | 6.0 ± 0.0 | 14.0 ± 1.0 | 100/50 | 50/50 | 2.5/2.5 | 20/20 | >100/>100 | 8.8 | 2.2 | 2.2 | 0.14 | 35 | 12.49 | 12.19 | 12.19 | 12.19 |
| Streptococcus mutans IB | 9.8 ± 0.3 | 7.4 ± 0.8 | 7.0 ± 0.0 | 8.5 ± 0.4 | 6.0 ± 0.0 | 9.3 ± 0.8 | 6.0 ± 0.0 | 15.5 ± 0.4 | 50/20 | 50/20 | 2.5/2.5 | 50/50 | >100/>100 | > 70 | 17.5 | 4.4 | 0.14 | 70 | 11.96 | 11.56 | 11.96 | 11.56 |
| Streptococcus mutans OMZ65 | 7.7± 0.8 | 7.0 ± 0.7 | 6.5 ± 0.0 | 9.8 ± 1.3 | 6.0 ± 0.0 | 11.0 ± 0.0 | 6.0 ± 0.0 | 15.8 ± 0.3 | 100/50 | 20/7.5 | 2.5/2.5 | 50/20 | >100/>100 | > 70 | 17.5 | 4.4 | 0.07 | > 70 | 12.49 | 12.19 | 11.79 | 11.36 |
| Streptococcus sobrinus B13 | 6.7 ± 0.3 | 6.7 ± 0.2 | 6.5 ± 0.0 | 9.0 ± 0.5 | 6.0 ± 0.0 | 9.5 ± 0.0 | 6.0 ± 0.0 | 16.3 ± 0.3 | 100/100 | 10/10 | 2.5/2.5 | 50/50 | >100/>100 | > 70 | n/a | 4.4 | 0.14 | 70 | 12.49 | 12.49 | 11.49 | 11.49 |
| Streptococcus salivarius ATCC8618 | 6.8 ± 0.3 | 6.8 ± 0.2 | 6.5 ± 0.0 | 8.8 ± 0.6 | 6.0 ± 0.0 | 9.3 ± 0.2 | 6.0 ± 0.0 | 12.8 ± 0.6 | 50/20 | 20/20 | 2.5/2.5 | 50/50 | >100/>100 | > 70 | n/a | 4.4 | 0.03 | 17.5 | 13.26 | 12.86 | 12.86 | 12.86 |
| Caries group sum of means* | 38.0d | 34.6e | 33.0f | 45.4c | 30.0g | 49.4b | 30.0g | 74.4a | 400/240 | 150/107.5 | 12.5/12.5 | 220/190 | >500 | |||||||||
| Porphyromonas gingivalis OMGS1740 | 10.5 ± 1.5 | 7.6 ± 1.3 | 6.5 ± 0.0 | 9.3 ± 0.9 | 6.0 ± 0.0 | 9.2 ± 0.6 | 6.0 ± 0.0 | 18.8 ± 0.8 | 10/7.5 | 20/7.5 | 2.5/7.5 | 50/50 | >100/>100 | n/a | n/a | n/a | n/a | n/a | 11.88 | 11.75 | 11.88 | 11.76 |
| Prevotella nigrescens ATCC33563 | 7.5 ± 1.5 | 7.5 ± 1.5 | 9.0 ± 0.0 | 13.5 ± 0.5 | 6.0 ± 0.0 | 9.5 ± 0.0 | 6.0 ± 0.0 | 20.7 ± 0.5 | 20/20 | 100/20 | 5/5 | 50/20 | >100/>100 | n/a | n/a | n/a | n/a | n/a | 11.95 | 11.95 | 12.65 | 11.95 |
| Prevotella intermedia ATCC25611 | 9.3 ± 0.3 | 7.5 ± 1.0 | 9.0 ± 2.5 | 10.3 ± 0.8 | 6.0 ± 0.0 | 9.8 ± 0.3 | 6.0 ± 0.0 | 19.7 ± 0.5 | 20/20 | 20/20 | 5/2.5 | 50/50 | >100/>100 | n/a | n/a | n/a | n/a | n/a | 12.20 | 12.20 | 12.20 | 12.20 |
| Agregatibacter actinomycetemcomitans ATCC29522 | 6.8± 0.8 | 7.1 ± 1.4 | 6.5 ± 0.0 | 7.5 ± 0.0 | 6.0 ± 0.0 | 7.8 ± 0.6 | 6.0 ± 0.0 | 16.4 ± 0.4 | 5/5 | 10/2.5 | 2.5/2.5 | 20/20 | >100/>100 | 70 | > 70 | 8.8 | 0.07 | > 70 | 11.45 | 11.45 | 12.06 | 12.06 |
| Fusobacterium nucleatum ATCC10953 | 6.5± 0.0 | 7.2 ± 0.8 | 6.5 ± 0.0 | 13.5 ± 0.5 | 6.0 ± 0.0 | 9.0 ± 0.5 | 6.0 ± 0.0 | 18.3 ± 1.3 | 20/50 | 5/5 | 2.5/2.5 | 50/20 | >100/>100 | 4.4 | 70 | 4.4 | 0.03 | 70 | 12.46 | 12.86 | 11.86 | 11.86 |
| Periodontal group sum of means * | 30.1e | 36.9d | 37.5d | 54.1b | 30.0e | 45.3c | 30.0e | 94.0a | 75/102.5 | 155/55 | 17.5/20 | 220/160 | >500 | |||||||||
| RANKING based on sum of means | 4 | 5 | 6 | 2 | 7 | 3 | 7 | 1 | 4 | 2 | 1 | 3 | 5 | |||||||||
*Denote letters, in agar disc diffusion test, indicate significant difference (one-way ANOVA with Tukey-Kramer post hoc test; p<0.05) amongst agents for sum of means for caries and periodontal group, same letter indicates no difference
**Active Cl+: bacterial cell (log10 mol/CFU)
n/a, results unavailable due to technical difficulties