Fig. 2.

Effects of 3-OHB administration on isolated perfused hearts. A, average traces from isolated hearts showing changes in left ventricular systolic pressure during perfusion with Krebs-Henseleit buffer added 3 mM Na-3-OHB or 3 mM extra NaCl (n = 10–12). Data points in panel A are also represented in panel B. B, 3-OHB-induced changes in cardiac parameters measured from isolated perfused hearts ex vivo (n = 9–12) 10 minutes after buffer change. Values after 20 minutes are reported in Supplementary Fig. S1. Bars and symbols represent mean±SEM. In addition to the relative changes illustrated by the bars, the values at the base of the bars indicate the absolute changes. In panel B, responses to Na-3-OHB and equimolar NaCl were compared by unpaired two-tailed Student’s t-tests. Abbreviations: CFR coronary flow rate; HR heart rate; LVDP left ventricular developed pressure; LVSP left ventricular systolic pressure