Table 1.

Bacterial strains used in this study.

Strain/plasmid	Relevant genotype (*)	Source/reference
S. Typhimurium
SV5015	SL1344 hisG+	63
MD5052	∆mrdA (∆PBP2)	19
MD2576	∆PBP2SAL	19
MD5054	PBP2SAL-3xFLAG	This work
MD2259	PBP3SAL-3xFLAG::KmR	21
MD5510	∆mrdA PBP2SAL-3xFLAG	This work
MD5549	∆mrdA pAC-6xHIS-PBP2SAL	This work
MD5550	∆mrdA pAC-6xHIS-PBP2SAL S326A	This work
MD5057	∆mrdA pFUS	This work
MD5058	∆mrdA pFUS-PBP2	This work
MD5059	∆mrdA pFUS-PBP2SAL	This work
MD6296	PBP2SAL-3xHA::CmR	This work
MD6297	PBP2SAL-3xHA::CmR dacA-3xFLAG::KmR	This work
MD6298	PBP2SAL-3xHA::CmR dacB-3xFLAG::KmR	This work
MD6299	PBP2SAL-3xHA::CmR dacC-3xFLAG::KmR	This work
MD6300	PBP2SAL-3xHA::CmR dacD-3xFLAG::KmR	This work
MD6505	PBP2SAL-3xHA::CmR fepA-3xFLAG::KmR	This work
MD5577	∆mreB:: KmR	This work
MD5578	∆mreC:: KmR	This work
MD5579	∆mreD:: KmR	This work
MD5580	∆mreCD:: KmR	This work
MD5581	∆mreBCD:: KmR	This work
MD5583	∆PBP2SAL ∆mreB:: KmR	This work
MD5584	∆PBP2SAL ∆mreBCD:: KmR	This work
MD5585	∆mrdA ∆mreB:: KmR	This work
MD5588	∆mrdA ∆mreBCD:: KmR	This work
MD2591	∆mrcA (∆PBP1a)	This work
MD2569	∆mrcB (∆PBP1b)	This work
MD6264	∆mrdA ∆PBP1a:: KmR	This work
MD6274	∆mrdA ∆PBP1b:: KmR	This work
MD3201	∆PBP2SAL ∆PBP1a:: KmR	This work
MD6283	∆PBP2SAL ∆PBP1b:: KmR	This work
MD5064	PBP2SAL-3xFLAG PBP3SAL-3xFLAG	19
MD5516	∆mrdA PBP2SAL-3xFLAG PBP3SAL-3xFLAG::KmR	This work
MD5098	∆PBP2SAL PBP3SAL-3xFLAG::KmR	This work
E. coli
BW25141	ΔlacZ4787(::rrnB-3) Δ(phoB-phoR)580 λ- galU95 ΔuidA3::pir+ recA1 endA9(del-ins)::FRT rph-1 Δ(rhaD-rhaB)568 hsdR514	66
MD6291	BW25141 pSU314::3xHA	This work

*All S. Typhimurium strains listed are isogenic and derivates of parental strain SV5015.