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. 2023 Sep 9;14:5567. doi: 10.1038/s41467-023-41279-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Force-directed layouts of 4633 epicardial cells displaying timepoint, annotated cluster, and origin. b A dot plot displaying select genes enriched within epicardial clusters, including AV epicardial-derived cells (EPDCs) and mesenchyme. Size of the dot represents proportion of the cluster that expresses each gene. Colour indicates level of expression. c Force-directed layouts of 4633 epicardial cells displaying pseudotime and differentiation (diff.) potential. Arrow indicates differentiation potential maximum where AV EPDCs are specified. d Gene trends along pseudotime show bifurcation between epicardial and AV mesenchymal trajectories during the epicardial-to-mesenchymal transition. AV atrioventricular, epi epicardium, mes mesenchyme, STM septum transversum mesenchyme. See also Supplementary Fig. 5 and Supplementary Data 2.