Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 9;14:5554. doi: 10.1038/s41467-023-41306-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Representative confocal microscopy images of tdTomato⁺ cells and immunostaining of glucagon (GCG), and insulin (INS) during the secondary transition at E15.5. Note the increased number of tdTomato⁺ cells without a detectable expression of INS or GCG (arrowheads). b Quantification of tdTomato⁺ area without GCG and INS expression in the central section of pancreas with the largest pancreatic footprint. Data are presented as mean ± SEM; (n = 31 tdTomato⁺ areas/Control from 4 pancreases and 25 tdTomato⁺ areas/Neurod1ST from 4 pancreases); unpaired two-tailed t test. Source data are provided as a Source Data file. c Relative mRNA expression of glucagon (Gcg), insulin 1 (Ins1), and insulin 2 (Ins2) by RT-qPCR using RNA extracted from E15.5 pancreases. Data are presented as mean ± SEM; (n = 8 pancreases/genotype); unpaired two-tailed t test, Source data are provided as a Source Data file. d Representative confocal microscopy images of tdTomato⁺ cells and immunostaining of INS, C-peptide 1 and C-peptide 2 in P0 pancreatic tissue sections. Scale bars, 50 µm. ns, not significant.