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. Author manuscript; available in PMC: 2023 Sep 10.
Published in final edited form as: Nano Lett. 2021 Feb 26;21(5):2272–2280. doi: 10.1021/acs.nanolett.1c00118

Figure 1.

Figure 1.

Adsorption of ACE2 sensing proteins to (GT)6-SWCNTs to form nanosensor constructs. (a) Schematic depiction of ACE2-SWCNT nanosensor formation, with sensing protein ACE2. (b) ACE2-SWCNT complexation was observed as quenching of the intrinsic SWCNT near-infrared fluorescence following 1 h incubation of 6.25 mg/L ACE2 with 2.5 mg/L (GT)6-SWCNTs (final concentrations). (c) ACE2-SWCNT construct demonstrated time-stable quenched fluorescence. All fluorescence measurements were obtained with 721 nm laser excitation. Gray bars represent the standard error between experimental replicates (N = 3). (d) Adsorption of ACE2 on the SWCNT surface led to (GT)6 desorption, tracked by Cy5-labeled ssDNA following addition of 6.25 mg/L ACE2 with 2.5 mg/L Cy5-(GT)6-SWCNTs (final concentrations). The increase in Cy5-(GT)6 fluorescence from the initial quenched state on the SWCNT serves as a proxy for ACE2 adsorption. Shaded error bars represent the standard error between experimental replicates (N = 3).