TABLE 5.
Reliability of species differentiation at PCR laboratories
Laboratorya | Identification method(s) | No. of samples with species:
|
No. of false positives | % Accuracy of reported results | % of positive samples correctly identified (n = 28) | |
---|---|---|---|---|---|---|
Correctly identified | Incorrectly identified | |||||
P1 | SSPb | 25 | 2 | 1 | 89 | 89 |
P2 | RFLP | 20 | 3 | 0 | 87 | 71 |
P3 | SSP | 19 | 0 | 1 | 95 | 68 |
P4 | SSP | 17 | 0 | 0 | 100 | 61 |
P6c | SSP, RFLP | 10 | 0 | 1 | 91 | 36 |
Avg | 18.2 | 1 | 0.6 | 92 | 65 |
Laboratory P5 did not determine species.
SSP, species-specific primers.
Enterocytozoon bieneusi-specific primers were used exclusively.