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. 2023 Jul 24;15(9):e16431. doi: 10.15252/emmm.202216431

Figure 5. SLF2 deficiency leads to loss of CLSPN and key DDR and cell cycle regulators.

Figure 5

  1. TMT‐based MS results of chromatin fractions from SU‐DHL‐5 control and SU‐DHL‐5 SLF2 KO cells. Volcano plot depicting depleted chromatin‐associated proteins. Significant hits are shown by red dots (depleted in SLF2 KO cells). Proteins belonging to the DDR and cell cycle regulation are labeled. Experiments were performed in triplicates.
  2. Immunoblot analysis of human SU‐DHL‐5 control and SLF2 KO cell lines with the indicated antibodies.
  3. Quantification of the CLSPN western blots in (B). Protein expression of CLSPN was normalized to β‐Tubulin expression. Data are presented as mean ± SD of n = 3 independent experiments. P‐value determined by unpaired t‐test.
  4. qPCR analysis of CLSPN mRNA expression of SU‐DHL‐5 control and SLF2 KO cell lines. Data are presented as mean ± SD of n = 3 independent experiments. P‐value determined by unpaired t‐test.
  5. Immunoblot analysis of human SU‐DHL‐5 control and SLF2 KO whole cell fractions with the indicated antibodies.
  6. Immunoblot analysis of human chromatin fractions of SU‐DHL‐5 control and SLF2 KO cell lines with the indicated antibodies.

Source data are available online for this figure.