Figure 1. Characterization of the TAZ ^G197V mouse model.

• A
  Representative pictures of mouse body size, dissected heart, and testis from 12 weeks old wild type (WT) and TAZ^G197V mice. Bar, 0.5 cm.
• B
  Body weight of animals at indicated age. Mean ± SEM, n = 9, 2Way ANOVA: *P < 0.05, **P < 0.01, ****P < 0.0001.
• C
  Western blot analysis of isolated mouse heart mitochondria from 12‐week‐old animals.
• D
  Survival of indicated mice presented as percent of total number of mice analyzed. n = 60 (per genotype), median survival of TAZ^G197V mice is 24.5 weeks, Mantel‐Cox test: ****P < 0.0001.
• E
  Quantification of neutrophils, percent of white blood cells (left), white blood cells (middle), and red blood cells (right) of 12‐week‐old mice. Mean ± SEM, n = 6, unpaired t‐test: ****P < 0.0001, ns (non‐significant).
• F–H
  Echocardiography of wild type and TAZ^G197V mice. (F) Ejection fraction from short axis expressed in %, (G) Fractional shortening in %, and (H) Longitudinal systolic strain expressed in %. Mean ± SEM, n = 9, 2‐way ANOVA: *P < 0.05.
• I
  Picrosirius red staining of histology sections of hearts from WT and TAZ mutant mice.
• J
  qPCR analysis of ANP (left) and BNP (right) from total mRNA isolated from heart lysates of 20‐week‐old mice. Mean ± SEM, n = 5 for ANP, n = 4 for BNP, unpaired t‐test: *P < 0.05, ***P < 0.001.

Source data are available online for this figure.