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. 2023 Aug 3;15(9):e17399. doi: 10.15252/emmm.202317399

Figure EV3. TAZ‐deficient MEF cells display reduced fatty acid oxidation.

Figure EV3

  1. Western blot analysis (left) and quantification (right) of CPT1 and CPT2 protein levels in WT and TAZKO MEF cell lysate normalized to β‐tubulin. Mean ± SEM, n = 4, unpaired t‐test: **P < 0.01, ***P < 0.001.
  2. Oxygen consumption rate of WT and TAZ MEF cells in media supplemented with palmitate‐BSA, basal, oligomycin, CCCP, and antimycin/rotenone treated (left). ETO, etomoxir. Quantification of maximal respiratory capacity (right). Mean ± SEM, n = 3, unpaired t‐test: ****P < 0.0001.
  3. Extracellular acidification rate, presentation of basal rate, addition of glucose, oligomycin, and 2 deoxyglucose (2‐DG). Quantification of Glycolysis and Glycolytic capacity of the cells is presented on the right. Mean ± SEM, n = 6, unpaired t‐test: *P < 0.05.