Table 2.
Effect of a SFC fed for 9 weeks on caloric intake, body and liver weight as well as markers of liver damage, inflammation and glucose metabolism in wild-type and TNFα−/− mice.
| wild-type mice |
TNFα−/− mice |
|||
|---|---|---|---|---|
| Control | SFC | Control | SFC | |
| Caloric intake (kcal/g/bw) | 0.39 ± 0.00 | 0.47 ± 0.01a,c | 0.38 ± 0.00 | 0.46 ± 0.01a,c |
| Body weight (g) | 27.7 ± 1.0 | 29.2 ± 0.7 | 27.3 ± 0.3 | 28.1 ± 0.5 |
| Absolute body weight gain (g) | 3.2 ± 0.7 | 6.0 ± 0.5a | 4.2 ± 0.2 | 5.5 ± 0.2a |
| Liver weight (g) | 1.2 ± 0.1 | 1.7 ± 0.1a,c | 1.3 ± 0.0 | 1.6 ± 0.0a,c |
| Liver:body weight ratio (%) | 4.5 ± 0.1 | 5.9 ± 0.2a,c | 4.6 ± 0.0 | 5.8 ± 0.1a,c |
| Steatosis (NAS) | 0.11 ± 0.0 | 1.20 ± 0.2a,c,d | 0.25 ± 0.1 | 0.50 ± 0.1a |
| Inflammation (NAS) | 0.21 ± 0.1 | 0.88 ± 0.2d | 0.22 ± 0.1 | 0.45 ± 0.1 |
| ALT (U/L) | 13.2 ± 1.4 | 20.6 ± 1.5a,c | 15.7 ± 0.7 | 20.3 ± 1.1a,c |
| Fasting blood glucose (mg/dl) | 124.4 ± 5.4 | 165.3 ± 7.2a,c | 136.3 ± 5.5 | 158.4 ± 5.9a |
| aSma mRNA expression | 100.0 ± 29.2 | 97.7 ± 27.5 | 123.2 ± 25.7 | 132.2 ± 20.6 |
| Col1a1 mRNA expression | 100.0 ± 8.9 | 229.9 ± 70.8 | 143.7 ± 17.2 | 133.8 ± 16.2 |
Data are shown as mean ± SEM, n = 6–8; ap < 0.05 compared to C-fed wild-type mice, cp < 0.05 compared to C-fed TNFα−/− mice, dp < 0.05 compared to SFC-fed TNFα−/− mice. aSma, alpha smooth muscle actin; ALT, alanine aminotransferase; Col1a1, collagen type I alpha1; C, control diet; NAS, NAFLD activity score; SFC, sucrose-, fat-, and cholesterol-rich diet.