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. 2023 May 5;29(10):3081–3093. doi: 10.1111/cns.14250

FIGURE 4.

FIGURE 4

Effects of gthrombin on the differentiation and neurite elongation of PC12 cells. (A and B) Effects of 10 μg/mL gthrombin on the undifferentiated PC12 cells following treatment for 24 h. (C and D) Effects of 10 μg/mL gthrombin on the neurite elongation of differentiating PC12 cells. The cells were stimulated with 10 μg/mL gthrombin for 24 h, followed by incubation at 50 ng/mL NGF for 48 h. (E and F) Effects of 10 μg/mL gthrombin on the neurite elongation of differentiated PC12 cells following treatment for 24 h. (G) and (H) are statistical analyses of (C and D) and (E and F) from five fields each 20 cells. (I–N) The effects of equivalent hthrombin on the undifferentiated (I and J), differentiating (K and L), and differentiated PC12 cells (M and N). (O) and (P) are statistical analyses of (K and L) and (M and N) from 5 to 14 fields each 20 cells. Data are represented as mean ± SEM (p < 0.05). Scale bars, 20 μm in (A and B), and (I–N); 50 μm in (C and D); 25 μm in (E and F). (Q) Differentiated PC12 cells were treated with 5 μM Fluo4‐AM for 30 min, followed by the addition of 10 μg/mL of gthrombin. The fluorescence intensity of intracellular free calcium concentration in PC12 cells was recorded every 5 s. (I) The original record of changes in calcium concentration in individual neurons (by ratio of gthrombin/PBS).