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. 2023 Apr 26;29(10):2787–2799. doi: 10.1111/cns.14230

FIGURE 4.

FIGURE 4

Apigenin/BDNF synergistically protects Aβ‐induced toxicity in neuronal cells. (A) Different doses of Aβ25‐35 were applied onto retinoic acid pre‐treated SH‐SY5Y cells, or cultured cortical neurons at DIV4, for 24 h (left). Cultures were pretreated with different concentrations of BDNF for one hour, followed by 24 h of Aβ exposure at 3 μM (right). Cell viability was measured by MTT assay. (B) BDNF and apigenin were co‐applied to cultures from Aβ25‐35 at 3 μM. Cell viability was measured by MTT assay. (C) The protective effect of apigenin and BDNF against Aβ25‐35 was tested by the staining of PI and Hoechst 33342 (left). The density of PI‐positive cells was quantified (right). Values are expressed as percentage of control, or the fold of change, compared to the control, in mean ± SEM, n = 4–6. (*) p < 0.05 against Aβ‐treated control.