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Immunoblot analysis of crude and partially purified antigen (fraction 9) obtained by HPLC. Crude (lanes 1 and 3) and partially purified (lanes 2 and 4) antigens were probed with anti-B. equi immune serum (lanes 1 and 2) and MAb BEG3 (lanes 3 and 4). MW, molecular weight markers (in thousands).
