REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
barcoded West Nile virus (bcWNV), lineage I, strain 3356	Colorado State University, Microbiology department	N/A
Deposited data
Sequencing files (.fastq)	Mendeley Data: https://doi.org/10.17632/88xx6596wz.1https://doi.org/10.17632/s7kthpysb7.1https://doi.org/10.17632/556zktntym.1.	N/A
Experimental models: Cell lines
Vero cells	ATCC	CVCL_0059
CT (Culex tarsalis derived) cells	Aaron Brault, Centers for Disease Control and Prevention	CVCL_0F44
Experimental models: Organisms/strains
Culex tarsalis mosquitoes	USA, CA	N/A
Culex quinquefasciatus mosquitoes	USA, FL	N/A
Aedes aegypti mosquitoes	Mexico, Poza Rica	N/A
Oligonucleotides
WNV_ENV_F70 - TCAGCGATCTCTCCACCAAAG	IDT	N/A
WNV_ENV_R70 - GGGTCAGCACGTTTGTCATTG	IDT	N/A
WNENV-prob - FAM/TGCCCGACC/ ZEN/ATGGGAGAAGCTC/3|ABkFQ/	IDT	N/A
ID_cDNAWNV_7374_Rev - GTCTCGTGGGCTCG GAGATGTGTATAAGAGACAGNNNNNNNNN cagtGCCATCCACTACAGCGTTCT	IDT	N/A
5'_ID_Primer_Rev - GTCTCGTGGGCTCGGA GATGTGTAT	IDT	N/A
5'_ID_WNV_7171_For - TCGTCGGCAGCGTCA GATGTGTATAAGAGACAGNNNNTTCCCCTT CGTCGATGTTGG	IDT	N/A
2ND round FP (N5XX) - AATGATACGGCGAC CACCGAGATCTACAC[i5]TCGTCGGCAGCGTC	IDT	N/A
2ND round RP (N7XXX) - CAAGCAGAAGACG GCATACGAGAT[i7]GTCTCGTGGGCTCGG	IDT	N/A
Software and algorithms
Original code used for used for data processing, analysis, and visualization.	https://github.com/fitz-meyer/bcWNV_analysis.git	R - SCR_001905 GitHub - SCR_002630
Statistical analyses	R version 4.2.2 Prism version 9.4.1	R - SCR_001905 Prism -SCR_002798