FIG. 2.

Fragment analysis results for sensitivity of solid-phase purification with a fivefold dilution series of RNA competitor 1. 1a to 1d, 57, 12, 2.3, and 0.5 RNA copies (size, 89 bp), respectively, bound onto oligo(dT)₂₅ Dynabeads and then subjected to RT-PCR; 2a to 2d, 57, 12, 2.3, and 0.5 RNA copies, respectively, bound onto oligo(dT)₂₅ Dynabeads and then eluted from the solid phase and subjected to RT-PCR in solution; 3a to 3d, the same dilutions of RNA 1 analyzed directly by RT-PCR in solution; M, dye-marked ladder (50 and 100 bp).