PCR cycle test for RNA competitors and HIV-1 RNA target; Amplification kinetics of inner PCR for a low-copy-number mixture of RNA competitors coanalyzed by RT-PCR with 3,000 copies of HIV-1 RNA target. Configuration of the RNA competitors was as follows: RNA 1, 89 bp and 40 copies; RNA 2, 100 bp and 250 copies; RNA 3, 125 bp and 1,250 copies; and RNA 4, 136 bp and 5,000 copies.