Figure 3.

Atazanavir treatment and lamin A/C R482W mutation alter cell rheological behavior

UNT: untreated control cells (control); DMSO: control cells incubated with DMSO (AZN control); AZN: control cells treated with atazanavir; T2D: cells from a patient with type 2 diabetes; M,K: cells from patients with FPLD2 carrying the LMNA R482W mutation.

(A) Entry time in 6x6-μm² constrictions T_e (median ±95% CI, Confidence Interval).

(B and C) Fits of tongue length l as a function of time t (t = 0 defined at cell contact with constriction), for (B) UNT, M and K cells, and (C) DMSO and AZN cells. Solid curves with symbols indicate the median fits; dashed upper and lower curves delineate the 95% CIs; the curves are plotted using median and CI values from Table 1 in Equation 1.

(D–F) Fit-extracted rheological parameters: long-time viscosity η₂ (D), short-time elastic modulus E (E), and viscosity η₁ (F). Medians ±95% CI are displayed. Significant differences: AZN vs. DMSO cells (¤), FPLD2 and T2D vs. UNT cells (∗). Number of experiments: N ≥ 3; n: number of analyzed cells (A); n': number of fitted curves (D–F).