Fig. 8.

Comparison of InflectSSP outputs with “TPP” program and integration of “melt coefficient”. 1 h thapsigargin experiment used (n = 3). A, number of significant proteins identified through InflectSSP and “TPP”. Melt curves for SERCA2A (target of thapsigargin) by InflectSSP (B). C, melt coefficient formula that incorporates attributes of melt shift to quantify significance of a melt shift. D, distribution of melt coefficients for proteins with p-value <0.05 and ≥ 0.05. E, melt coefficient distribution with associated relevant GO terms for proteins with melt p-value <0.05. F, melt curves for protein of interest, MNK1, identified in (E). TPP, thermal proteome profiling.