FIG. 5.

Nested PCR amplification of DNA from nine positive fox fecal samples; ethidium bromide staining of 10 μl of PCR products after 1.5% agarose gel electrophoresis showed the specific 250-bp band (a). The reaction products were analyzed by Southern transfer and hybridized with internal oligonucleotide E.multi.1. labeled at the 5′ end with digoxigenin (b). Lanes A, positive control; lanes B, C, D, E, F, G, H, I, and J, positive fox fecal samples; lanes K, negative control.