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. 2023 Aug 26;66:102863. doi: 10.1016/j.redox.2023.102863

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Low-dose LPS alleviates early brain injury after SAH via modulating microglial M1/M2 polarization by FOXO1/IL-10/IL-10R1 axis. (A) The mRNA and protein levels of FOXO1 and IL-10 were detected by qRT-PCR and Western blot, respectively. (B) The expression of FOXO1 was detected by IF. (C) Brain water content was assessed by wet-dry weight method. (D) BBB permeability were measured by EB permeability assay. (E) Apoptosis of prefrontal cortical and hippocampal neurons was detected by TUNEL assay. (F) The secretion of cytokines was detected by ELISA assay. (G) The expression of IBA1 and M1/M2 markers were detected by IF with quantitative analysis. (H) The protein levels of SOD and Gpx were determined by Western blot. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Fig. 4 — Low-dose LPS alleviates early brain injury after SAH via modulating microglial M1/M2 polarization by FOXO1/IL-10/IL-10R1 axis. (A) The mRNA and protein levels of FOXO1 and IL-10 were detected by qRT-PCR and Western blot, respectively. (B) The expression of FOXO1 was detected by IF. (C) Brain water content was assessed by wet-dry weight method. (D) BBB permeability were measured by EB permeability assay. (E) Apoptosis of prefrontal cortical and hippocampal neurons was detected by TUNEL assay. (F) The secretion of cytokines was detected by ELISA assay. (G) The expression of IBA1 and M1/M2 markers were detected by IF with quantitative analysis. (H) The protein levels of SOD and Gpx were determined by Western blot. *, P < 0.05; **, P < 0.01; ***, P < 0.001.