Figure 3.

Effects of Arg2 TSBs in human MDMs on pro- and anti-inflammatory markers

(A) CCL2 was analyzed by RT-PCR using TBP as the control and graphed as F.O.C. in MDMs transfected with TSB-NC, TSB-155, and TSB-3202, in the presence or absence of LPS stimulation (n = 5). (B) Supernatants were analyzed by ELISA for CCL2 and graphed as a percentage relative to NC-TSB (n = 6). (C) IL1B pro-inflammatory gene expression was analyzed by RT-PCR using TBP as the control and graphed as F.O.C. (n = 5). The anti-inflammatory associated genes (D) CCL18 and (E) MRC1 (CD206) were analyzed by RT-PCR using TBP as the control and graphed as F.O.C. (4 ≤ n ≤ 5). (F) CD206 was analyzed in TSB-treated cells stimulated with LPS by western blot using GAPDH as the loading control. Densitometry analysis was performed on CD206 western blots and normalized to GAPDH and graphed as relative expression to the NC-TSB with LPS (n = 5). Error bars are representative of the SEM. Statistical analysis was performed on using a one-way ANOVA with Dunnett’s multiple comparison test on all graphs. ns, not statistically significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.