TABLE 3.
Comparison of results between neutralization and IFA methods
| Neutralization assay result | No. of isolates identified by IFA with monoclonal antibodies
|
||||
|---|---|---|---|---|---|
| Poliovirus | Coxsackievirus | Echovirus | IFA negative | Indeterminate | |
| Polioviruses (n = 95) | 68 | 0 | 3 | 22 | 2a |
| Coxsackievirus type A (n = 8) | 0 | 1 | 5 | 2 | 0 |
| Coxsackievirus type B (n = 26) | 0 | 22 | 2 | 2b | 0 |
| Echoviruses, in blendc (n = 107) | 0 | 0 | 99 | 7 | 1 |
| Echoviruses, others (n = 54) | 0 | 0 | 8 | 47 | 0 |
| Enterovirus type 71 (n = 1) | 0 | 0 | 0 | 1 | 0 |
a
One isolate stained with both coxsackievirus and echovirus antibody blends. The second isolate stained with both poliovirus and echovirus antibody blends.
b
Coxsackievirus types B2 and B4.
c
Echovirus serotypes 4, 6, 9, 11, 30, and 34; sensitivity, 93%; specificity, 90%.