Figure 2. Neuron Locations and Their Positional Variability.

(A) Neuron locations and variability, in the retrovesicular ganglion, taken from electron micrographs of three adult hermaphrodites N2S, N2T, and N2U (Hall and Altun, 2007; White et al., 1986).

(B) An example of substantial positional variability. The OLL left (OLLL) and right (OLLR) neurons, within a single animal, should share equivalent positions. Instead they show substantial anterior-posterior displacement relative to each other. The transgenic reporters and their pseudo colors are noted on the figure.

(C and D) Canonical neuron locations (filled circles displaying the NeuroPAL colors) and their positional variability (encircling ellipses with matching colors) for all ganglia, as determined by NeuroPAL (otIs669, strain OH15262) (see Table S3 for data). Positional variability is shown as the 50% contour for neuronal location (measured as a Gaussian density distribution), sliced within a 2D plane (Methods S1). We show both the left-right and dorsal-ventral planes to provide a 3D estimation of positional variability. (C) Left, right, and ventral views of the head neuron positions. OLLR exhibits over twice the positional variability of OLLL in its anterior-posterior axis, echoing the displacement seen with the non-NeuroPAL transgene in (B). (D) Left, right, and ventral views of the tail neuron positions.

See also Table S3 and Methods S1.