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. Author manuscript; available in PMC: 2023 Sep 11.
Published in final edited form as: Cell. 2020 Dec 29;184(1):272–288.e11. doi: 10.1016/j.cell.2020.12.012

Figure 5. Mutant Analysis of the Conserved Transcription Factors PAG-3/Gli and EOR-1/PLZF.

Figure 5.

(A and B) Alterations in NeuroPAL coloring reveal neurons with altered fate in pag-3(−) mutant backgrounds: the AVE pre-motor interneuron gains blue-color expression, the PVR interneuron loses all green-color expression, and the VA and VB motor neurons are missing entirely. Statistical bar graph colors in (B) are sampled from the images in (A).

(C) PAG-3/Gli expression as assessed with a fosmid reporter (wgIs154): ADA*, ALM, AQR*, AVD*, AVE*, AVF, AVH*, AVJ*, AVM, BDU, DVC*, I1*, I2*, I6*, PLM, PQR*, PVC*, PVM, PVQ*, PVR*, PVW*, RID*, RIG, RMG*, VA11–12, and URY* (*previously unpublished expression).

(D) EOR-1/PLZF has broad, likely ubiquitous, expression, as assessed with a fosmid reporter (wgIs81).

(E–G) The NeuroPAL blue reporters in RMED/V are ggr-3, pdfr-1, and unc-25.

(E and F) NeuroPAL exhibits total loss of its RMED/V blue-color expression in eor-1(−) mutant backgrounds but retains panneuronal marker expression (shown in white), indicating the preservation of RMED/V neuronal fate.

(G) RMED/V fate analysis in eor-1(−) mutants with unc-25 (otIs514) and unc-47 (otIs565) reporter transgenes.

See Table S5 for data.