Skip to main content
. Author manuscript; available in PMC: 2024 Aug 17.
Published in final edited form as: Mol Cell. 2023 Aug 17;83(16):3010–3026.e8. doi: 10.1016/j.molcel.2023.07.017

Figure 7. FAM120A RNA binding is essential for lipogenesis and tumor growth.

Figure 7.

(A) Schematic of de novo fatty acid synthesis pathway.

(B, C) Heatmap of fatty acid levels. LC-MS analysis was performed on LAM cells transfected with siRNAs targeting FAM120A or control (B) and H1299 cells stably expressing shRNAs targeting FAM120A or control (C) with overnight serum starvation. N = 3.

(D-H) Cell proliferation analysis of LAM (D), H1299 (E), DLD1 (F), MCF7 (G), and LNCaP (H) cells stably expressing shRNAs targeting FAM120A or control. N = 3. Data are represented as mean ± SD.

(I-M) Crystal violet (CV) analysis of LAM (I), H1299 (J), DLD1 (K), MCF7 (L), and LNCaP (M) cells stably expressing shRNAs targeting FAM120A or control. Cells were grown in lipoprotein-deficient serum (LPDS)-containing media supplemented with lipoprotein (25 μg/ml), palmitate-albumin (10 μM, palmitate), oleate-albumin (50 μM, oleate), or fatty acid-free albumin (25 μM, control). The graph shows the quantified absorbance of solubilized CV stain. N = 3. Data are represented as mean ± SD.

(N-O) Xenograft tumor growth assay of H1299 cells stably expressing shNTC or shFAM120A with FAM120A WT or ∆RBD. N ≥ 4. Data are represented as mean ± SEM. p-value (*) was calculated between shNTC and shFAM120A+Empty. p-value (#) was calculated between shFAM120A+Empty and shFAM120A+WT. Data are represented as mean ± SD. ***p < 0.001, #p < 0.05, ##p < 0.01, and ###p < 0.001.

See also Supplemental Figure S7.