Table 2. The results of data analysis for two models made in blocks for each population separately.

Temp (°C)	Isoline	Population	Block	Slope.ev	Slope.anc	Slope.diff	Slope.ratio	Slope. p	Fisher slope. p	Mean.ev	Mean.anc	Mean.diff	Mean.ratio	Mean. p	Fisher mean. p
20	Iz8	K02	3	0.082	0.065	0.017	1.261	0.439		0.190	0.175	0.015	1.089	0.877
						12	0.106	0.061	0.045	1.735	0.102		0.541	0.254	0.288	2.133	0.038*
					16	0.040	0.052	−0.012	0.772	0.474		0.100	0.124	−0.024	0.808	0.725
				K12	4	0.111	0.112	−0.002	0.987	0.930		0.367	0.356	0.012	1.033	0.929
						12	0.106	0.061	0.044	1.722	0.180		0.339	0.254	0.086	1.337	0.588
					16	0.069	0.052	0.017	1.332	0.274		0.217	0.124	0.093	1.751	0.244
				K25	4	0.110	0.112	−0.003	0.975	0.836		0.450	0.356	0.094	1.266	0.453
						12	0.097	0.061	0.036	1.583	0.223		0.299	0.254	0.045	1.179	0.734
					16	0.039	0.052	−0.013	0.755	0.461		0.099	0.124	−0.025	0.800	0.718
				K54	4	0.118	0.112	0.006	1.049	0.731	0.193	0.500	0.356	0.144	1.406	0.279	0.580
						12	0.109	0.061	0.048	1.778		0.051		0.332	0.254	0.078		1.308	0.548
			16		0.070	0.052	0.017	1.334	0.350	0.165		0.124	0.042	1.336	0.619
		Iz6	K60	6	0.099	0.106	−0.007	0.933	0.753		0.324	0.322	0.002	1.005	0.99
					11	0.106	0.031	0.075	3.408	0.001*		0.292	0.073	0.219	4.000	0.038
				K28	6	0.112	0.106	0.007	1.065	0.651	0.374	0.404	0.322	0.082	1.255	0.511	0.341
			11		0.052	0.031	0.021	1.661	0.184	0.151		0.073	0.078	2.068	0.205
		Iz9	K29	2	0.053	0.018	0.035	2.937	0.060		0.156	0.05	0.106	3.121	0.110
						11	0.111	0.029	0.082	3.810	0.001*		0.383	0.450	−0.067	0.852	0.682
	17			0.040	0.033	0.007	1.224	0.562		0.101	0.088	0.012	1.139	0.816
24	Iz6	E01	6	0.047	0.028	0.019	1.674	0.091	0.017*	0.162	0.076	0.087	2.146	0.099	0.074
					9	0.024	0.009	0.015		2.750	0.026*	0.056	0.017	0.039		3.265	0.141
				E02	6	0.058	0.028	0.030	2.052	0.030*	0.010*	0.154	0.076	0.079	2.043	0.198	0.000*
						9	0.039	0.009	0.031	4.594		0.040*		0.106	0.017	0.088		6.147	0.085
					14	0.025	0.008	0.017	3.000	0.179		0.222	0.028	0.194	8.000	0.000*
				E03	7	0.043	0.013	0.030	3.320	0.004*	0.006*	0.117	0.034	0.083	3.412	0.064	0.130
						9	0.022	0.009	0.013	2.515		0.047*		0.049	0.017	0.032		2.868	0.200
					15	0.051	0.043	0.008	1.194	0.594		0.133	0.094	0.039	1.412	0.560
				E05	7	0.034	0.013	0.021	2.643	0.011*	0.014*	0.083	0.034	0.048	2.406	0.179	0.194
					9	0.018	0.009	0.010	2.132	0.182		0.045	0.017	0.028	2.618	0.269
				E06	5	0.043	0.014	0.029	3.090	0.008*		0.138	0.042	0.096	3.302	0.036*
						14	0.008	0.008	0.000	1.000	1.000		0.017	0.028	−0.011	0.600	0.426
					15	0.015	0.043	−0.028	0.355	0.027		0.039	0.094	−0.056	0.412	0.249
				E08	7	0.031	0.013	0.018	2.417	0.096	0.052	0.080	0.034	0.046	2.338	0.246	0.203
					8	0.052	0.021	0.031	2.496	0.096		0.134	0.050	0.084	2.671	0.208
				E09	5	0.028	0.014	0.014	2.019	0.087		0.097	0.042	0.055	2.324	0.094
						8	0.023	0.021	0.002	1.107	0.834		0.081	0.050	0.031	1.622	0.407
		15	0.021		0.043	−0.022	0.484	0.099		0.050	0.094	−0.044	0.529	0.387
24	Iz6	E12	5	0.060	0.014	0.046	4.314	0.002*		0.176	0.042	0.134	4.206	0.031*
				15	0.042	0.043	−0.001	0.968	0.921		0.117	0.094	0.022	1.235	0.709
		Iz8	E14	3	0.047	0.034	0.013	1.390	0.254	0.000*	0.138	0.096	0.042	1.434	0.442	0.000*
						10	0.049	0.003	0.047		17.800	0.000*		0.305	0.006		0.300	54.982	0.000*
					13	0.032	0.008	0.024	3.841		0.025*	0.068	0.022	0.046	3.068		0.225
				E17	4	0.022	0.074	−0.051	0.303	0.003		0.055	0.300	−0.245	0.183	0.002
					10	0.014	0.003	0.011	5.000	0.054		0.028	0.006	0.022	5.000	0.238
				E18	4	0.060	0.074	−0.014	0.816	0.534		0.218	0.300	−0.082	0.727	0.385
						10	0.080	0.003	0.078	28.955	0.000*		0.433	0.006	0.427	77.909	0.000*
			13		0.024	0.008	0.016	2.879	0.199		0.203	0.022	0.180	9.114	0.000*
		Iz9	E34	2	0.080	0.028	0.052	2.886	0.000*		0.289	0.061	0.228	4.736	0.003*
	13			0.015	0.021	−0.006	0.733	0.649		0.033	0.042	−0.008	0.800	0.832

Notes.

Slope.ev

slope estimate for a given evolved population in a given block

slope.anc

slope estimate for the ancestral population in the same block

slope.diff

difference between the former and the latter

slope.ratio

ratio of the former to the latter (analogously for means)

slope. p

P value for the interaction term in model 1

mean. p

P value for the interaction term in model 2

Statistically significant values (P < 0.05) are marked with: asterisk for positive coefficient estimates or italic font for negative coefficient estimates. Underscore marking block number means that in that block, the population it applies to was thawed from a different generation than in the previous block(s) (cf. Table 1 for details). Bolded fonts are marking populations that in all blocks had positive slope and mean differences (slope.diff & mean.diff). For those populations, the P values (for means and slopes separately) were combined using the Fisher’s method. The resulting combined P values are represented in columns Fisher slope. p and Fisher mean. p for slope. p and mean. p accordingly.