Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 1;23(5):825–837. doi: 10.17305/bb.2022.8846

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Sun et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 4. — SPD inhibits ferroptosis pathway in vivo and in vitro. According to the analysis of proteomic results, diabetic cardiomyopathy is involved in ferroptosis pathway and related detections were performed: (A and B) Representative western blot of ODC and SSAT in comparison with β-actin expression in mice and cardiomyocytes; (C and D) The expression of ACSL-4 and GPX-4 in cardiomyocytes and mice; (E) Mitochondrial membrane potential (Δ ψm) was measured using JC-1 staining and photos were taken using fluorescence microscopy in cardiomyocytes treated with or without HG/SPD; (F–I) Heart tissues were collected in 12 weeks to measure iron, GPX-4 activity, GSH level, and GSH/GSSG ratio in the four groups. *P < 0.05 vs WT/Control group; ^#P < 0.05 vs T2D/HG group (n ≥ 3). SPD: Spermidine; ODC: Ornithine decarboxylase; SSAT: SPD/spermine N1-acetyltransferase; GPX-4: Glutathione peroxidase 4; WT: Wild-type; T2D: Type 2 diabetes; GSH: Glutathione; HG: High glucose.