Table 3.
Primer sequences for qRT-PCR
| Gene | Primer sequence (5ʹ—3ʹ) | Size (bp) | Accession No. |
|---|---|---|---|
| ZO-1 | Forward: ACGCTGGTGAAATCAAGGAAGAA Reverse: AGGGACATTCAACAGCGTGGC |
255 | XM_013093747.1 |
| ZO-2 | Forward: ACAGTGAAAGAAGCTGGCGTAG Reverse: GCTGTATTCCCTGCTACGGTC |
131 | XM_005019888.2 |
| OCLN | Forward: CAGGATGTGGCAGAGGAATACAA Reverse: CCTTGTCGTAGTCGCTCACCAT |
160 | XM 013109403.1 |
| CLDN-1 | Forward: TCATGGTATGGCAACAGAGTGG Reverse: CGGGTGGGTGGATAGGAAGT |
179 | XM_013108556.1 |
| CLDN-2 | Forward: CTCCTCCTTGTTCACCCTCATC Reverse: GAACTCGCTCTTGGGTTTGTG |
160 | XM_005009661.2 |
| JAM3 | Forward: TGCATAGCAACAAACGACGC Reverse: GTAGCCCCTTCTGTAGGCAC |
159 | XM_027444289.2 |
| MUC2 | Forward: GGGCGCTCAATTCAACATAAGTA Reverse: TAAACTGATGGCTTCTTATGCGG |
150 | XM_005024513.2 |
| TFF2 | Forward: CTGTCATCCTTGTTGTAGCCCTCA Reverse: GATTCCTGGGTGACCACAGTTCTT |
113 | XM_005030468.2 |
| IgA | Forward: TCGCTCAAGGAACCCATCGT Reverse: GCGGGACCACGAGAACTTCA |
174 | U27222.1 |
| LYZ | Forward: TAACACGCAGGCTACAAACCG Reverse: TTCCATCGCTGACAATCCTCTT |
194 | XM_005008880.2 |
| MT1 | Forward: GACTCCCAGGACTGCCCTT Reverse: ACGACGTGCATTTGCAGTTT |
85 | XM_027466320.2 |
| ZIP3 | Forward: CGCCCTCTGCAATTCCTTCG Reverse: TCTCAGCCACCGGGTAGTCC |
134 | XM_027471837.2 |
| ZIP6 | Forward: CAAGTCGGTTGCTCGGTTAG Reverse: GTCAACAGAGCCTGAGTGCC |
231 | XM_013107243.4 |
| ZIP8 | Forward: ACACAAACCACCATCATCCAG Reverse: TGGGTTACTTCCTCCTGTGC |
123 | XM_038177855.1 |
| ZIP9 | Forward: AGTGAGTGAGATGCAGCACG Reverse: AGGACAAGGGAAACACCAAT |
117 | XM_027457903.2 |
| ZIP10 | Forward: GCGACGGCTTAGCAATAGGA Reverse: GACCGCAAAGATCCACAAGG |
251 | XM_038182345.1 |
| ZIP12 | Forward: GCTGATGGCTTGGTAATAGG Reverse: ATGGGTCAGTTGAAACAGAAA |
223 | XM_013093603.4 |
| ZIP14 | Forward: AGGAGTTCCCGCACGAGTTG Reverse: ACGATGCCGAAAGCCAAGC |
127 | XM_038166959.1 |
| ZnT1 | Forward: TGGAGATGCCTTAGGTTCGG Reverse: GTGGTTATTGACGCAGGGATTA |
112 | XM_027453027.2 |
| ZnT4 | Forward: GTGGATCGCATTAAGGAAGACT Reverse: CCTTGAACTGAACTTCCTCCC |
157 | XM_005019478.4 |
| ZnT6 | Forward: ACTCCAGACAACTCCACCTCAT Reverse: CAATGCGTATAATCGGTTAGTGA |
208 | XM_027453882.2 |
| ZnT7 | Forward: ATAGGCGTAAGCATTGTTCCAC Reverse: GCTACCAGTAATTTCAAAGTCCCT |
197 | XM_027462589.2 |
| ZnT9 | Forward: TTCCTCAAAGACCCAATCCA Reverse: TGCCTCTACATCCGACCTCA |
219 | XM_038178939.1 |
| ZnT10 | Forward: AGATGGCACAAGGCAAACAC Reverse: CGATCCCAACAATGAGGACA |
172 | XM_013105385.4 |
| β-actin | Forward: AGAAATTGTGCGTGACATCAA Reverse: GGACTCCATACCCAAGAAAGAT |
227 | XM_013108556.1 |
ZO-1, zonula occludens-1; ZO-2, zonula occludens-2; OCLN, occluding; CLDN1, claudin 1; CLDN2, claudin 2; JAM3, junctional adhesion molecule 3; MUC2, mucin 2; TFF2, trefoil factor 2; IgA, immunoglobulin A; LYZ, lysozyme; MT1, metallothionein; ZIP3, zinc transporter SLC39A3; ZIP6, zinc transporter SLC39A6; ZIP8, zinc transporter SLC39A8; ZIP9, zinc transporter SLC39A9; ZIP10, zinc transporter SLC39A10; ZIP12, zinc transporter SLC39A12; ZIP14, zinc transporter SLC39A14; ZnT1, zinc transporter SLC30A1; ZnT4, zinc transporter SLC30A4; ZnT6, zinc transporter SLC30A6; ZnT7, zinc transporter SLC30A7; ZnT9, zinc transporter SLC30A9; ZnT10, zinc transporter SLC30A10.