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(a) Time-dependent in vivo fluorescence imaging of a HepG-2 tumor mouse model (7 days and 35 days after subcutaneous implantation of HepG-2 cells) and after intratumoral injection of the FBN-1 probe. Fluorescence imaging of (b) 7-day-old excised tumors with ~6 mm diameters, or (c) 35-day-old excised tumors with ~14 mm diameters, dissected from six independent HepG-2-tumor-carrying mice models. (d) Quantitative detection of NTR in solid tumors of different sizes with ELISA.
