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. 2023 Sep 11;6(12):e202302021. doi: 10.26508/lsa.202302021

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© 2023 Mrestani et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — (A) Overview of the Drosophila melanogaster rim locus on chromosome III and illustration of genomic targeting strategy. #1 and #2 mark positions of the gRNA probes. (B) Location and size of the rim gene fragment excised by CRISPR/Cas9 and replaced by φC31-mediated integration to generate rim^rescue-Znf (gray), rim^V5-Znf (blue), and rim^HA-Znf (magenta) alleles. UTR, untranslated region. (C) Drosophila RIM protein domain positions: Rab3/zinc finger (Znf), PDZ, C₂A, PxxP, and C₂B domains and position of the N-terminal epitope tag (V5-Znf or HA-Znf) is indicated.