Author response image 1. Effects of tetrodotoxin (TTX) and picrotoxin (PTX) on the mRNA levels of DCV cargos in (Δ)Cre-expressing neurons.

(A) mRNA levels of DCV cargo genes upon treatment of (Δ)Cre-expressing cultures with either 1 μM TTX or 50 μM PTX. (B) mRNA levels of DCV cargo genes upon treatment of (Δ)Cre-expressing cultures with a combination of 1 μM TTX and 50 μM PTX. Data are shown as fold changes (FC) relative to the averaged ΔCre-untreated control levels. n=3 culture preparations/ genotype. Dots represent individual cultures; bars are geometric means. Log2FC were analyzed using two-way ANOVA with Holm-Šídák's multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001.