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. 2023 Aug 28;25(9):1359–1368. doi: 10.1038/s41556-023-01213-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, Western blot of RBFOX2 in K562 cells transfected with shNS or RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). b, Percentages of cells with CD61⁺ staining in K562 cells transduced with shNS or RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). n = 10000 cells examined over 4 biologically independent experiments. c, Western blot showing RBFOX2 expression in control group (shNS+EV, control shRNA (shNS) with empty vector (EV)), RBFOX2 KD group (shRBFOX2 + EV, RBFOX2 KD with EV) and RBFOX2 rescue group (shRBFOX2 + RBFOX2, RBFOX2 KD with RBFOX2 overexpression) in K562 cells. d-e, Relative expression of RBFOX2 during hematopoietic cell differentiation into different lineages based on GSE42519 (d) and GSE24759 (e) datasets. The sample size shown in (d) and (e) are biologically independent human samples. Data are presented as mean values +/− SEM. Two-sided P values by Student’s t-test.

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