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. 2023 Sep 11;14:5579. doi: 10.1038/s41467-023-41364-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Mice were subcutaneously infected or not with 300 stage L3 larvae of Nippostrongylus brasiliensis. 7 days post-infection lungs were collected, and cells were isolated and analysed via flow cytometry for cytokine production, and T_RM cells. a Numbers of activated (CD44⁺) CD4⁺ T cells producing indicated cytokines, p_{(Non-infected vs Nippostrongylus brasiliensis)}: IFN-γ, p = 0.001749; IL-17, p = 0.002537; IL-4, p = 0.000048; IL-13, p = 0.000729, and b representative flow cytometry plots (non-infected n = 6, infected n = 9, N = 3). c Numbers of activated CD8⁺ T cells producing indicated cytokines and d Representative flow cytometry plots of cytokine production in CD8⁺ T cells (non-infected n = 6, infected n = 9, N = 3). e, f Foxp3^WT mice were assessed for e percentage and f numbers of chemokine receptors CXCR3, ST2 (IL-33R) and CCR6 expression on T_REG cells, p_{(Non-infected vs Nippostrongylus brasiliensis)}: CXCR3, p = 0.014118; ST2, p = 0.023778; CCR6, p = 0.028885, (non-infected n = 6, infected n = 9, N = 3). g, h CD69⁺KLRG1⁻ CD8⁺ T_RM cell g percentage of total CD8⁺ T cells, p_{(Non-infected vs Nippostrongylus brasiliensis)} = 0.0004, and h number, p_{(Non-infected vs Nippostrongylus brasiliensis)}= = 0.0016, in non-infected (control) and infected mice (non-infected n = 6, infected n = 9, N = 3). i–l Foxp3^WT mice received CD8^CD45.1 T cells intravenously, one day prior to infection. 14 days post-infection, lung cells were analysed via flow cytometry for i total number of CD8^CD45.1 T cells, CD69⁺KLRG1⁻ CD8⁺ T_RM cell j percentage and k numbers, and l representative flow plot, within the CD8^CD45.1 T-cell population (n = 7, N = 3). m, n Comparison of transferred CD8^CD45.1 T-cell number in the (m) spleen, p_{(Influenza vs Nippostrongylus brasiliensis)} = 0.0012, or (n) lungs, p_{(Influenza vs Nippostrongylus brasiliensis)} = 0.0002, of mice infected with Influenza and N. brasiliensis (Influenza n = 9, N. brasiliensis n = 7, N = 3). 2-sided Mann–Whitney analysis was applied to compare the differences between groups. Data is presented as bars of mean ± SEM with single data points.