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. 2023 Sep 12;12:224. doi: 10.1038/s41377-023-01245-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The size and working distance comparison between LED of LEC and mercury lamp. LEC is mounted inside a microscope filter cube with the excitation light collimated by a Fresnel lens and reflected by a dichroic mirror into the objective lens. b The assembly of LEC components designed for fluorescence microscope. c LECs were mounted in fluorescence microscope