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. 2023 Sep 11;14:5573. doi: 10.1038/s41467-023-41331-5

Fig. 4. Inducible multiplex gene knockouts in mammalian cells.

Fig. 4

a Schematic of the gene knockout by introducing A•T to G•C base conversions (highlighted in red) at multiple splice donor sites using DAP-MBE and ABEs. The consensus sequence of splice donors is GT. The target adenines are located on the antisense strand and are emboldened. DAP-MBE: drive-and-process multiplex base editing array. b, c Protein expression of b B2M gene and c CD46 gene in HEK293T cells transfected with sgRNAs or DAP-MBE array targeting splice donors and sABE v3.22 or ABE8e. The percentages of B2M- or CD46-positive cells were quantified using antibody-based FACS analysis. n = 2 in b in the group transfected with ABE8e and sgRNA 1. n = 3 in all other groups. Dots represent individual biological replicates, and bars represent mean ± s.d. b, c use the unpaired two-tailed t-test; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. P-values in b from left to right are 0.0502 (ns), 0.6709 (ns), 0.0709 (ns), and 0.0252 (**). P-values in c from left to right are 0.1024 (ns), 0.7595 (ns), 0.0286 (*), and 0.0018 (**).