TABLE 1.

Nucleotide sequences of PCR primers

Test	Primer paira	Nucleotide sequence	Size of PCR product (bp)	Reference
Diagnostic PCR	A1	5′-GGGAAAACGACAATTGC-3′	732	19
	A2	5′-GTACAATGCGGCCGTTA-3′
	B1	5′-ATGGGAAGCCGATAGTC-3′	635	19
	B2	5′-GATTTCGTTCCTCGACC-3′
	C1	5′-GGTATCAAGGAAACCTC-3′	822	19
	C2	5′-CTTCCGCCATCATAGCT-3′
	D1	5′-CTCCTACGATTCTCTTG-3′	439	19
	D2	5′-CGAGCAAGACCTTTAAG-3′
Transposon mapping of structural genes	VanR	5′-AGCGATAAAATACTTATTGTGGA-3′	645	28
	VanR1	5′-CGGATTATCAATGGTGTCGTT-3′
	VanS	5′-AACGACTATTCCAAACTAGAAC-3′	1,094	28
	VanS1	5′-GCTGGAAGCTCTACCCTAAA-3′
	VanH	5′-ATCGGCATTACTGTTTATGGAT-3′	943	28
	VanH1	5′-TCCTTTCAAAATCCAAACAGTTT-3′
	VanA	5′-ATGAATAGAATAAAAGTTGCAATAC-3′	1,029	28
	VanA1	5′-CCCCTTTAACGCTAATACGAT-3′
	VanY	5′-ACTTAGGTTATGACTACGTTAAT-3′	866	28
	VanY1	5′-CCTCCTTGAATTAGTATGTGTT-3′
	Orf1A	5′-AGGGCGACATATGGTGTAACA-3′	844	28
	Orf1A1	5′-GGGCGACGGTACAACATCTT-3′
	Orf1B	5′-TGGTGGCTCCTTTTCCCAGTTC-3′	1,007	28
	Orf1B1	5′-CGTCCTGCCGACTATGATTATTT-3′
	Orf1C	5′-ACCGTTTTTGCAGTAAGTCTAAAT-3′	1,066	28
	Orf1C1	5′-AAACGGGATTTAGAAATAGTTAAT-3′
	Orf2D	5′-CCATTTCTGTATTTTCAATTTATTA-3′	925	28
	Orf2D1	5′-CATAGTTATCACCCTTTCACATA-3′
	Orf2E	5′-TTGCGGAAAATCGGTTATATTC-3′	540	28
	Orf2E1	5′-AGCCCTAGATACATTAGTAATT-3′
Transposon mapping of intergenic regions	VanXY1	5′-AATAGCTATTTTGATTTCCCCGTTA-3′	543	6
	VanXY2	5′-TCCTGAGAAAACAGTGCTTCATTAA-3′
	VanSH1	5′-TAGGGTAGAGCTTCCAGCGATTGC-3′	311	6
	VanSH2	5′-CTCATCCTGCTCACATCCATAAACA-3′
	VanYZ1	5′-GTTTCCCGGATCAACACATACTA-3′	336	6
	VanYZ2	5′-CCCAGTAGCAGTAAATGGAGTCA-3′

^aPrimers D1 and D2 are specific for the vanC2 gene. Orf1 is transposase, and Orf2 is resolvase.