Fig. 1.

CBX4 deletion induces LUAD and causes a worse prognosis in Kras^G12D mice model. a A scheme for Adeno-Cre virus treatment for Kras^G12D and Cbx4^−/−, Kras^G12D mouse model. b Representative photographs of lung tissue of Kras^G12D and Cbx4^−/−, Kras^G12D mice after inhalation of Adeno-Cre for 18 weeks. Arrows indicate tumor lesions. Scale bar: 1 cm. c Tumor nodules’ number count of lung tissue of Kras^G12D (n = 8) and Cbx4^−/−, Kras^G12D mice (n = 8) after inhalation of Adeno-Cre for 18 weeks. d Representative H&E staining in lung sections from Kras^G12D and Cbx4^−/−, Kras^G12D mice. Scale bar: 100 μm (left), 10 μm (right). e Tumor nodules’ number count and tumor area data of lung tissue from Kras^G12D and Cbx4^−/−, Kras^G12D mice after inhalation of Adeno-Cre for 18 weeks. f Representative immunohistochemical staining of Ki67 in lung sections from Kras^G12D and Cbx4^−/−, Kras^G12D mice. Scale bar: 500 μm (up), 200 μm (down). g Representative immunohistochemical staining of cleaved-Caspase3 in lung sections from Kras^G12D and Cbx4^−/−, Kras^G12D mice. Scale bar: 500 μm (up), 200 μm (down). h Quantitative analysis of Ki67 positive cell number in IHC staining of lung sections from Kras^G12D and Cbx4^−/−, Kras^G12D mice. i Quantitative analysis of cleaved Caspase3 positive cell number in IHC staining of lung sections from Kras^G12D and Cbx4^−/−, Kras^G12D mice. j Kaplan–Meier survival curves of Kras^G12D mice group (n = 10) and Cbx4^−/−, Kras^G12D mice group (n = 10) after Adeno-Cre treatment. Data are shown as means ± SEM. **p < 0.01, ***p < 0.001