Fig. 3.
Typical selected Cbx4−/−, KrasG12D clones gain cell proliferation and clone formation abilities in vitro which cannot be rescued by ectopic CBX4. a Genotype (with or without Cre recombinase) of selected Cbx4−/−, KrasG12D clones. Wild-type (W), heterozygote (T) and mutant (M) type were used as control. b MTT assay of 5 selected Cbx4−/−, KrasG12D clones and the control (KrasG12D MEFs). c, d Colony formation and transformation abilities of five selected Cbx4−/−, KrasG12D clones were shown by soft agar assay and the quantitative analysis. e MTT analysis to show cell proliferation with or without ectopic expression of CBX4 with the control of KrasG12D cells. f, g Colony formation ability of selected Cbx4−/−, KrasG12D cells (clone1 and clone3 representatively) with or without ectopic expression of CBX4 is determined by soft agar assay compared with the control of KrasG12D MEFs. Scale bar: 200 μm (left) and 50 μm (right). Data are shown as means ± SEM. Ns p > 0.05, **p < 0.01, ***p < 0.001