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. 2023 Sep 11;6:929. doi: 10.1038/s42003-023-05293-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Screen of all purified TTAs using the TTA-ADH coupled assay on 3. The experiment was performed in triplicate with each replicate as an individual point. Error bars represent standard deviations. b Apparent l-Thr K_M and k_cat measurements for TTAs that exhibited activity greater than or equal to ObiH calculated using non-linear regression. Parenthetical values represent the 95% confidence interval. c Heatmap showing initial rates for six active TTAs against multiple aromatic aldehyde substrates. d Multi-sequence alignment of the predicted conserved catalytic residues for the six active TTAs. e Superimposed structure and predicted structure illustrating the Tyr55-Pro71 loop region of ObiH compared to the predicted equivalent region for PbTTA. ObiH is represented in green with the loop region highlighted in yellow with the PLP highlighted in gray indicating the region of the active site. The PbTTA is indicated in blue with the corresponding loop region highlighted in pink.