Figure 4.

Overview of gut microbiota analyses in stool samples of patients before and after initiation of ICI treatment. (A-C) Beta-diversity across gut microbioma samples did not significantly differ between (A) pretherapeutic (blue) and late time-point (red), (B) responders (blue) vs. non-responders (red) and (C) patients who were still alive (blue) vs. deceased (red) at 6 months of ICI therapy. (D, E) Taxonomic binning at order (D) and family level (E) shows comparable relative abundance of different taxa across all 37 pretherapeutic samples. (F-K) Comparison of baseline to late time-point relative abundance of bacterial orders (F, I), families (G, J) and the most frequent genus (H, K) show non-significant shifts of bacterial composition between both time points.