Fig. 4.

CMP reveals altered spatiotemporal expression patterns for free amino acids in congenital cataracts. (A) Representative CMP pictures of WT and K6W-Ub lenses collected at E15 (top), P1 (middle), and P30 (bottom) and stained against alanine, cysteine, aspartate, lysine, glutamate, glutamine, glycine, arginine, glutathione, valine, taurine, and serine. (B) Metabolic signatures of WT and K6W-Ub lenses collected at P1 and colored according to metabolic classes derived from K-means clustering. (C) Partial least squares regression analysis discriminated metabolomes of WT (green) and K6W-Ub (red) lenses at all ages. (D) Variable importance in projection (VIP) scores for different amino acids responsible for the separation in the regression plot. All amino acids were higher in WT than K6W-Ub except for cysteine. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)