Fig. 7.

Downregulated proteins in K6W-Ub lenses associated with glutathione and amino acids metabolism. (A–B) Representative images of immunofluorescence (A) in E15 and P1 WT and K6W-Ub lenses and (B) Western blotting of lysates of P1 WT and K6W-Ub lenses using antibodies against γ-glutamylcyclotransferase (GGCT). (C–D) Representative images of immunofluorescence (C) in E15 and P1 WT and K6W-Ub lenses and (D) Western blotting of lysates of P1 lenses using antibodies against glutathione s-transferase pi 1 (GSTP1). (E–F) Representative immunoblot against heme oxygenase 1 (HMOX1) (E), guanidinoacetate N-methyltransferase (GAMT) (E), and phosphoglycerate mutase 2 (PGAM2) (F) in P30 WT and K6W-Ub lenses. Actin-normalized densitometric quantitation is shown for each sample. For all Western blots, two biological replicates are shown.