Table 3.

Gene Expression Alternation of CASC2, AKT and GAPDH Genes under Treatment with Thiosemicarbasone (Ni and Cu) and TMZ, in Different Concentrations and Times

Drug name	Gene/LncRNA	Concentration	Incubation time	Expression change level
Thiosemicarbazone (Cu)	CASC2	17.5	24h	0.564
		20	24h	234
		22.5	24h	0.383
		25	24h	0.114
		15.5	48h	0.12
		17	48h	0.207
		18.5	48h	0.024
		20	48h	0.389
		15	72h	2.895
		16	72h	2.334
		17	72h	2.497
		18	72h	2.668
Thiosemicarbazone (Ni)	CASC2	100.5	24h	2.283
		104	24h	2.634
		107.5	24h	0.067
		111	24h	0.037
		57	48h	0.616
		61	48h	0.051
		65	48h	0.121
		69	48h	0.001
		46	72h	0.465
		48	72h	0.032
		50	72h	0.665
		52	72h	0.654
TMZ	CASC2	100	24h	2.041
		150	24h	2.075
		200	24h	2.268
		250	24h	5.934
		100	48h	1.304
		150	48h	1.899
		200	48h	1.219
		250	48h	2.406
		100	72h	4.075
		150	72h	1.264
		200	72h	2.383
		250	72h	3.373
Thiosemicarbazone (Cu)	AKT	17.5	24h	0.22
		20	24h	0.019
		22.5	24h	0.047
		25	24h	0.001
		15.5	48h	0.186
		17	48h	0.531
		18.5	48h	0.087
		20	48h	0.003
		15	72h	0.776
		16	72h	0.251
		17	72h	0.156
		18	72h	0.037
Thiosemicarbazone (Ni)	AKT	100.5	24h	4.452
		104	24h	3.9
		107.5	24h	3.669
Drug name	Gene/LncRNA	Concentration	Incubation time	Expression change level
Thiosemicarbazone (Ni)		111	24h	4.335
		57	48h	0.001
		61	48h	0
		65	48h	0.005
		69	48h	0
		46	72h	0.539
		48	72h	0.33
		50	72h	0.362
		52	72h	0.755
TMZ	AKT	100	24h	2.831
		150	24h	2.592
		200	24h	2.854
		250	24h	1.18
		100	48h	0.121
		150	48h	0.147
		200	48h	0.07
		250	48h	0.015
		100	72h	0.383
		150	72h	0.24
		200	72h	0.06
		250	72h	0.086