| Antibodies |
| anti- mouse CD8-APC/Cy7 |
Biolegend |
Cat# 100714; RRID: AB_312753 |
| anti-mouse CD69-PE |
Biolegend |
Cat# 104507; RRID: AB_313110 |
| anti-mouse PD-1-BV605 |
BioLegend |
Cat# 135219; RRID: AB_11125371 |
| anti-mouse LAG3-PE/Cy7 |
BioLegend |
Cat# 125226; RRID: AB_2715764 |
| anti-mouse CD366-BV421 |
BioLegend |
Cat# 134019; RRID: AB_2814028 |
| anti-mouse Annexin V-FITC |
BioLegend |
Cat# 640906; RRID: AB_2561292 |
| anti-mouse CD45-APC/Cy7 |
BioLegend |
Cat# 157203; RRID: AB_2876533 |
| anti-mouse CD3-PE |
BioLegend |
Cat# 100206; RRID: AB_312663 |
| anti-mouse CD8-AF700 |
BioLegend |
Cat# 100730; RRID: AB_493703 |
| anti-mouse CD69-BV421 |
BioLgend |
Cat# 104545; RRID: AB_2686969 |
| anti-mouse PD-1-PE/Cy7 |
BioLegend |
Cat# 109110; RRID: AB_572017 |
| anti-mouse LAG3-BV650 |
BioLegend |
Cat# 125227; RRID: AB_2687209 |
| Anti-mouse Annexin V-APC |
BioLegend |
Cat# 640941; RRID: AB_2616657 |
| anti-hCD19-APC |
Biolegend |
Cat# 392504; RRID: AB_2728416 |
| anti-human CD19 CAR-PE |
Acrobiosystems |
Cat#FM3-HPY53 |
| anti-human CD8-PE/Cy7 |
BioLegend |
Cat# 344750; RRID: AB_2687201 |
| anti-mouse CD4-APC/Cy7 |
BioLegend |
Cat# 100414; RRID: AB_312699 |
| anti-mouse CD19-PE |
BioLegend |
Cat# 115507; RRID: AB_313642 |
| anti-mouse NK1.1-APC |
BioLegend |
Cat# 108709; RRID: AB_313396 |
| Biotin anti-mouse CD3 |
BioLegend |
Cat# 100243; RRID: AB_2563946 |
| Alexa Fluor-594 streptavidin |
BioLegend |
N/A |
| Alexa Fluor-488 anti-mouse CD8 |
BioLegend |
Cat# 100723; RRID: AB_389304 |
| Rabbit anti-mouse ATF3 antibody |
Cell Signaling |
Cat#18665S |
| Anti-mouse SUMO1 |
Invitrogen |
Cat# 33–2400; RRID: AB_2533109 |
| VeriBlot for IP Detection Reagent HRP |
Abcam |
N/A |
| Goat anti-mouse HRP-conjugated antibody |
Cell Signaling |
Cat# 7076S |
| Rat anti-mouse β-tubulin antibody |
Cell Signaling |
Cat# 2146 |
| PE-anti-mouse H-2Kb bounds to SIINFEKL antibody |
BioLegend |
Cat# 141603; RRID: AB_10897938 |
| Recombinant Anti-Mesothelin antibody |
Abcam |
Cat# ab196235 |
| Bacterial and virus strains |
|
|
|
| Biological samples |
|
|
| Healthy human peripheral T cells |
Human Immunology Core at the University of Pennsylvania |
N/A |
|
|
|
| Chemicals, peptides, and recombinant proteins |
| Prostaglandin E2 |
Sigma |
Cat# P0409 |
| DMSO |
Sigma |
Cat# 20–139 |
| VEGF165 |
Sigma |
Cat# V5765 |
| 1,1’-dioctadecyl-3,3,3’,3’tetramethylindodicarbocyanine |
Biotium |
Cat# 60014 |
| Latrunculin A |
Sigma |
Cat# L5163 |
| 25-hydroxycholesterol |
Sigma |
Cat# H1015 |
| GW3965 |
Sigma |
Cat# G6295 |
| Golgi stop |
BD |
Cat# BDB554724 |
| Transwell Chambers |
Millipore |
Cat# CLS3422–48EA |
| DNase I |
Roche |
Cat# 10104159001 |
| Collagenase D |
Roche |
Cat# 11088882001 |
| Percoll |
Sigma |
Cat# 17–0891-01 |
| Lymphoprep™
|
Stem Cell |
Cat# 07851 |
| Recombinant human IL-2 |
Sigma |
Cat# I0523 |
| Ovalbumin (257–264) chicken |
Sigma |
Cat# S7951 |
| Anti-PD-1 |
Bio X cell |
Cat# BE0146 Clone: RMP1–14 |
| CFSE |
Biolegend |
Cat# 79898 |
| Dynabeads™ Mouse T-Activator CD3/CD28 |
Gibco |
Cat# 11453D |
| Dynabeads™ Human T-Activator CD3/CD28 |
Gibco |
Cat# 11132D |
| CountBright™ Absolute Counting Beads |
Invitrogen |
Cat# C36950
|
| Fetal Bovine Serum |
Hyclone |
Cat# SH30071.03 |
| High-Capacity RNA-to-cDNA™ Kit |
Applied Biosystems |
Cat# 4387406 |
| Gibco™ L-Glutamine |
Gibco |
Cat# 25–030-081 |
| Intracellular Fix & Perm Buffer set |
eBioscience |
Cat# 88–8824 |
| Lipofectamin2000 |
Invitrogen |
Cat# 52887 |
| Retronectin |
Takara |
Cat# T100B |
| RBC lysis buffer |
Biolegend |
Cat# 420302 |
| Critical commercial assays |
| ONE-Glo™ Luciferase Assay System |
Promega |
Cat# E6120 |
| Power SYBR Green PCR Master Mix |
Thermo Fisher |
Cat# 4367659 |
| SimpleChIP Plus Enzymatic Chromatin IP Kit |
Cell Signaling |
Cat# 9005S |
| RNeasy Kits |
Qiagen |
Cat# 74004 |
| EasySep™ Mouse CD8+ T Cell Isolation Kit |
Stem Cell |
Cat# 19853 |
| EasySep™ Mouse T Cell Isolation Kit |
Stem Cell |
Cat# 19851 |
| EasySep™ Mouse CD4+ T Cell Isolation Kit |
Stem Cell |
Cat# 19852 |
| Deposited data |
| Bulk RNA-sequence data |
This paper |
GEO: GSE190702
|
| Raw data |
This paper |
Data S1 |
|
|
|
| Experimental models: Cell lines |
| Human: Phoenix-ECO |
ATCC |
Cat# CRL-3214™
|
| Mouse: MC38 |
ATCC |
N/A |
| Mouse: MC38 OVA |
Cancer Cell, 2017, 31: 194–207 |
N/A |
| Mouse: MC38-OVA-Luciferase |
Nat Cancer 2022 May 30. doi: 10.1038/s43018–022- 00383–0 |
N/A |
| Mouse: Hepa1–6 |
ATCC |
Cat# CRL-1830™
|
| Mouse: B16F10 |
ATCC |
Cat# CRL-6475™
|
| Mouse: MH6499c4 |
Immunity 49, 178–193 |
N/A |
| Human: NALM6 |
ATCC |
Cat# CRL-3273™
|
| Human: EM-Meso-GFP-Luciferase |
Oncoimmunology, 2018, VOL. 7, NO. 3, e1395997 |
N/A |
| Mouse: NIH 3T3 |
ATCC |
Cat# CRL-1658™
|
| Experimental models: Organisms/strains |
| Mouse: WT C57BL/6 |
Jackson Laboratory |
Cat# 000664 |
| Mouse: Ch25h−/− C57BL/6 |
Jackson Laboratory |
Cat# 016263 |
| Mouse: Ch25hΔCD8 C57BL/6 |
This paper |
N/A |
| Mouse: Atf3ΔCD8 C57BL/6 |
This paper |
N/A |
| Mouse: OT1 C57BL/6 |
Jackson Laboratory |
Cat# 003831 |
| Mouse: Rag1−/− C57BL/6J |
Jackson Laboratory |
Cat# 002216 |
| Mouse: NSG NOD/ShiLtJ |
Jackson Laboratory |
Cat# 005557 |
| Oligonucleotides |
| Primers for q-PCR, see table S1
|
This paper |
N/A |
| Recombinant DNA (plasmid) |
| Lenti-p-EF1α-Meso-scFV |
10.1073/pnas.0813101106. |
N/A |
| Lenti-p-EF1α-Meso-scFV-T2A-Ch25h |
This paper |
N/A |
| Retro-p-CD19-scFV-BBZ-MSGV |
10.1016/j.cell.2021.08.004. |
N/A |
| Retro-p-CD19-scFV-BBZ-T2A-Ch25h MSGV |
This paper |
N/A |
|
|
|
| Software and algorithms |
| Prism 9 |
GraphPad Prism9 |
https://www.graphpad.com/
|
| FlowJo 7.6 version |
FlowJo |
https://www.flowjo.com/
|
| Image J |
Image J |
https://imagej.nih.gov/ij/
|
| Other |
