Figure 2.

Sirt3 maintains neuronal viability and reduces OGD/R-induced cell death. N2a cells were transfected with Ad-Ctrl or Ad-Sirt3, and then were subjected to OGD/R to simulate cerebral I/R injury in vitro. A. Cell viability was determined with an MTT assay. B. An LDH release assay was used to measure LDH levels in media from N2a cells. C, D. Trypan blue staining was used to observe the number of apoptotic cells after OGD/R. E-H. RNA was isolated from N2a cells, and the levels of Bax, Bad, Bcl-2 and c-IAP were measured. *p<0.05 vs. Ad-Ctrl group, #p<0.05 vs. OGD/R+Ad-Ctrl group.